Seed Stratification & Growth
Transferring Plates to Growth Chamber
- Notebook
- Mechanisms
---- Square petri dishes
---- Small round petri dishes
---- Rubberbands
6) Moving Plates from Refrigerator to Growth Chamber
| 6.1) | Remove agar plates containing planted seeds from refrigerator after stratification period. |
| 6.2) | Record all plate numbers removed from refrigerator in log book |
| 6.3) | To prepare plates for entry into the growth chamber, place in mechanisms. |
| 6.4) | Mechanisms are constructed by placing one half of a square Petri dish face down on counter then placing planted agar plate on top. Next, place half of a small round Petri dish on top of the plated agar plate, then place another planted agar dish on top, another small round Petri dish, and a third planted agar plate. Finally, place the other half of the square Petri dish face up on top of the third planted agar plate. Note: Make sure all labels and seed lines are aligned. Secure mechanism with two rubber bands also in line with labels and seeds. |
| 6.5) | Record plate numbers in growth chamber log book. |
| 6.6) | Place mechanisms in growth chamber following designated organization pattern. Let grow for designated time period. |